STED MICROSCOPY OF
SYNAPTIC FUNCTION

start_rizzoli

WELCOME to the homepage
of Prof. Dr. Silvio O. Rizzoli

The diffraction barrier has been particularly problematic for imaging synaptic vesicles, which are among the smallest known organelles (30-50 nm in diameter). They are located in small areas in the synapses (about 1 micron in diameter).

 

Our group takes advantage of increase imaging resolution provided by STED to investigate synaptic vesicle function, with an emphasis on synaptic vesicle recycling. Since STED microscopy also allows imaging of protein domains, the group aims at studying the patterning of protein domains in the synapse, in order to understand its molecular architecture.

Research

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Publications

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latest publications

Major projects

Early Endosome Dynamics

Major Projects EARLY ENDOSOME DYNAMICS This project expands the scope of our studies in a cell biology direction. We investigate fusion/docking and sorting/budding in early

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EM of Synaptic Vesicle Pools

Major Projects EM OF SYNAPTIC VESICLE POOLS The project relies heavily on the use of the photoconversion (or photo-oxidation) technique, in which fluorescent compounds are

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Live STED Microscopy

Major Projects LIVE STED MICROSCOPY The project was initiated in December 2007, and focuses on the application of super-resolution techniques in living preparations. In line

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The Neuron Nanomap

Research & Background THE NEURON NANOMAP In this project, which has started in April 2014, we generate a super-resolution image – a nanomap – of

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