Future and Emerging Technologies (FET-OPEN) 2020: 

High-throughput imaging of proteins using nanobodies

The conventional detection of proteins by imaging employs fluorophores or antibodies directed at individual proteins. However, this methodology cannot be upscaled and employed in a high-throughput omics approach. The EU-funded IMAGEOMICS project aims to address this limitation through affinity probes that bind to specific peptide sequences located in more than one protein. Scientists will develop nanobodies that bind to such peptides and use them to label biological samples. The IMAGEOMICS strategy will provide information on the whole proteome of cells and tissues, paving the way for improved diagnostics for various diseases.

Involved scientists:

Silvio O. Rizzoli & Felipe Opazo, Universitaetsmedizin Goettingen – Georg-August Universitaet Goettingen – Stiftung Oeffentlichen Rechts, Germany

Doron Gerber, Bar-Ilan University, Israel

Steffen Frey, NanoTag Biotechnologies GmbH, Germany

Ilaria Testa, KTH Royal Institute of Technology, Sweden