Prof. Dr. Silvio O. Rizzoli
Publications of Silvio O. Rizzoli
Microtubules as a versatile reference standard for expansion microscopy.
PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis.
Ca2+-triggered (de)ubiquitination events in synapses.
How energy determines spatial localisation and copy number of molecules in neurons.
MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity.
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO.
Nat Biotechnol. 2024 Oct 9:10.1038/s41587-024-02431-9. doi: 10.1038/s41587-024-02431-9.
The synaptic vesicle cluster as a controller of pre- and postsynaptic structure and function.
Membrane trafficking of synaptic adhesion molecules.
Morphological correlates of synaptic protein turnover in the mouse brain.
The maintenance of oocytes in the mammalian ovary involves extreme protein longevity.
Erythropoietin restrains the inhibitory potential of interneurons in the mouse hippocampus.
Subcellular protein turnover in human neural progenitor cells revealed by correlative electron microscopy and nanoscale secondary ion mass spectrometry imaging.
Protein nanobarcodes enable single-step multiplexed fluorescence imaging.
Mycorrhizal C/N ratio determines plant-derived carbon and nitrogen allocation to symbiosis.
Synapsin condensation controls synaptic vesicle sequestering and dynamics.
High-resolution analysis of bound Ca2+ in neurons and synapses.
Tracking cell turnover in human brain using 15N-thymidine imaging mass spectrometry.
Proteomic analysis of the human hippocampus identifies neuronal pentraxin 1 (NPTX1) as synapto-axonal target in late-stage Parkinson’s disease.
Age-dependent structural reorganization of utricular ribbon synapses.
The long-loop recycling (LLR) of synaptic components as a question of economics.
A distinct pattern of growth and RAC1 signaling in melanoma brain metastasis cells.
Heat denaturation enables multicolor X10-STED microscopy.
Visualizing proteins by expansion microscopy.
Shaib AH, Chouaib AA, Chowdhury R, Mihaylov D, Zhang C, Imani V, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawar N, Eilts J, Kang J, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO.
Preprint: BioRxiv. 30 March 2023. doi: 10.1101/2022.08.03.502284.
Trafficking proteins show limited differences in mobility across different postsynaptic spines.
IGF-1 receptor regulates upward firing rate homeostasis via the mitochondrial calcium uniporter.
An iodine-containing probe as a tool for molecular detection in secondary ion mass spectrometry.
Kabatas Glowacki S, Agüi-Gonzalez P, Sograte-Idrissi S, Jähne S, Opazo F, Phan NTN, Rizzoli SO.
Chem Commun (Camb). 2022 Jul 5;58(54):7558-7561. doi: 10.1039/d2cc02290g.
Editorial: Protein Phase Separation and Aggregation in (Patho)Physiology of Neurons.
Editorial: Molecular Nanomachines of the Presynaptic Terminal, Volume II.
Protein lifetimes in aged brains reveal a proteostatic adaptation linking physiological aging to neurodegeneration.
α-Synuclein in the Synaptic Vesicle Liquid Phase: Active Player or Passive Bystander?
A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry.
Extracellular Matrix Recycling as a Novel Plasticity Mechanism With a Potential Role in Disease.
The Synaptic Extracellular Matrix: Long-Lived, Stable, and Still Remarkably Dynamic.
White matter integrity in mice requires continuous myelin synthesis at the inner tongue.
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Exotic nuclear spin behavior in dendritic macromolecules.
A microRNA signature that correlates with cognition and is a target against cognitive decline.
Islam MR, Kaurani L, Berulava T, Heilbronner U, Budde M, Centeno TP, Elerdashvili V, Zafieriou MP, Benito E, Sertel SM, Goldberg M, Senner F, Kalman JL, Burkhardt S, Oepen AS, Sakib MS, Kerimolgu C, Wirths O, Bickeböller H, Bartels C, Brosseron F, Buerger K, Cosma NC, Fliessbach K, Heneka MT, Janowitz D, Kilimann I, Kleinedam L, Laske C, Metzger CD, Munk MH, Perneczky R, Peters O, Priller J, Rauchmann BS, Roy N, Schneider A, Spottke A, Spruth EJ, Teipel S, Tscheuschler M, Wagner M, Wiltfang J, Düzel E, Jessen F; Delcode Study Group, Rizzoli SO, Zimmermann WH, Schulze TG, Falkai P, Sananbenesi F, Fischer A.
Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons.
Brain erythropoietin fine-tunes a counterbalance between neurodifferentiation and microglia in the adult hippocampus.
Fernandez Garcia-Agudo L, Steixner-Kumar AA, Curto Y, Barnkothe N, Hassouna I, Jähne S, Butt UJ, Grewe K, Weber MS, Green K, Rizzoli S, Nacher J, Nave KA, Ehrenreich H.
Cell Rep. 2021 Aug 24;36(8):109548. doi: 10.1016/j.celrep.2021.109548.
A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines.
Human brain organoids assemble functionally integrated bilateral optic vesicles.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Schinzel F, Karch CM, Bao G, Gottardo M, Suren AA, Hescheler J, Nagel-Wolfrum K, Persico V, Rizzoli SO, Altmüller J, Riparbelli MG, Callaini G, Goureau O, Papantonis A, Busskamp V, Schneider T, Gopalakrishnan J.
Cell Stem Cell. 2021 Aug 12:S1934-5909(21)00295-2. doi: 10.1016/j.stem.2021.07.010. Online ahead of print.
Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry.
Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN.
Nanomaterials (Basel). 2021 Jul 10;11(7):1797. doi: 10.3390/nano11071797.
Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics.
Saal KA, Warth Pérez Arias C, Roser AE, Christoph Koch J, Bähr M, Rizzoli SO, Lingor P.
J Neurochem. 2021 May;157(4):1052-1068. doi: 10.1111/jnc.15274. Epub 2021 Jan 6.
Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging.
Lange F, Agüi-Gonzalez P, Riedel D, Phan NTN, Jakobs S, Rizzoli SO.
PLoS One. 2021 May 10;16(5):e0240768. doi: 10.1371/journal.pone.0240768. eCollection 2021.
Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity.
Agüi-Gonzalez P, Guobin B, Gomes de Castro MA, Rizzoli SO, Phan NTN.
ACS Chem Neurosci. 2021 Apr 26. doi: 10.1021/acschemneuro.1c00031. Online ahead of print.
Ablation of Vti1a/1b Triggers Neural Progenitor Pool Depletion and Cortical Layer 5 Malformation in Late-embryonic Mouse Cortex.
Sokpor G, Rosenbusch J, Kunwar AJ, Rickmann M, Tuoc T, Rizzoli SO, Tarabykin V, von Mollard GF, Krieglstein K, Staiger JF.
Neuroscience. 2021 Mar 26:S0306-4522(21)00153-6. doi: 10.1016/j.neuroscience.2021.03.021. Online ahead of print.
Presynaptic activity and protein turnover are correlated at the single-synapse level.
Jähne S, Mikulasch F, Heuer HGH, Truckenbrodt S, Agüi-Gonzalez P, Grewe K, Vogts A, Rizzoli SO, Priesemann V.
Cell Rep. 2021 Mar 16;34(11):108841. doi: 10.1016/j.celrep.2021.108841.
The vesicle cluster as a major organizer of synaptic composition in the short-term and long-term.
Reshetniak S, Rizzoli SO.
Curr Opin Cell Biol. 2021 Mar 8;71:63-68. doi: 10.1016/j.ceb.2021.02.007. Online ahead of print.
Monitoring mitochondrial translation in living cells.
Yousefi R, Fornasiero EF, Cyganek L, Montoya J, Jakobs S, Rizzoli SO, Rehling P, Pacheu-Grau D.
Mol Cell Proteomics. 2021 Feb 11:100061. doi: 10.1016/j.mcpro.2021.100061. Online ahead of print.
Protein phosphorylation in depolarized synaptosomes: Dissecting primary effects of calcium from synaptic vesicle cycling.
Silbern I, Pan KT, Fiosins M, Bonn S, Rizzoli SO, Fornasiero EF, Urlaub H, Jahn R.
J Neurosci. 2021 Feb 10;41(6):1157-1173. doi: 10.1523/JNEUROSCI.0921-20.2020. Epub 2020 Dec 11.
The mRNA-Binding Protein RBM3 Regulates Activity Patterns and Local Synaptic Translation in Cultured Hippocampal Neurons.
Sertel SM, von Elling-Tammen MS, Rizzoli SO.
J Neurosci. 2021 Feb 10;41(6):1157-1173. doi: 10.1523/JNEUROSCI.0921-20.2020. Epub 2020 Dec 11.
Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.
Dankovich TM, Rizzoli SO.
iScience. 2021 Feb 3;24(3):102134. doi: 10.1016/j.isci.2021.102134. eCollection 2021 Mar 19.
NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover.
Bonnin EA, Fornasiero EF, Lange F, Turck CW, Rizzoli SO.
BMC Mol Cell Biol. 2021 Jan 11;22(1):5. doi: 10.1186/s12860-020-00339-1.
Simple multi-color super-resolution by X10 microscopy.
Truckenbrodt S, Rizzoli SO.
Methods Cell Biol. 2021;161:33-56. doi: 10.1016/bs.mcb.2020.04.016. Epub 2020 May 27.
Correction: Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S , Schlichthaerle T , Duque-Afonso CJ , Alevra M , Strauss S , Moser T , Jungmann R , Rizzoli SO , Opazo F .
Nanoscale. 2020 Dec 23;12(48):24543. doi: 10.1039/d0nr90279a.
A minimalist model to measure interactions between proteins and synaptic vesicles.
Perego E, Reshetniak S, Lorenz C, Hoffmann C, Milovanović D, Rizzoli SO, Köster S.
Sci Rep. 2020 Dec 3;10(1):21086. doi: 10.1038/s41598-020-77887-1.
Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway.
Gürth CM, Dankovich TM, Rizzoli SO, D’Este E.
Sci Rep. 2020 Nov 25;10(1):20576. doi: 10.1038/s41598-020-77260-2.
Quantitative Synaptic Biology: A Perspective on Techniques, Numbers and Expectations.
Reshetniak S, Fernández-Busnadiego R, Müller M, Rizzoli SO, Tetzlaff C.
Int J Mol Sci. 2020 Oct 2;21(19):7298. doi: 10.3390/ijms21197298.
Novel Secondary Ion Mass Spectrometry Methods for the
Examination of Metabolic Effects at the Cellular and Subcellular Levels.
Bonnin EA, Rizzoli SO.
Front Behav Neurosci. 2020 Jul 20;14:124. doi: 10.3389/fnbeh.2020.00124. eCollection 2020.
A Comparative Analysis of the Mobility of 45 Proteins in the Synaptic Bouton.
A Nanobody-Based Fluorescent Reporter Reveals Human α-Synuclein in the Cell Cytosol.
Nat Commun. 2020 Jun 1;11(1):2729. doi: 10.1038/s41467-020-16575-0.
Author Correction: WDR1 Is a Novel EYA3 Substrate and Its Dephosphorylation Induces Modifications of the Cellular Actin Cytoskeleton.
Mentel M, Ionescu AE, Puscalau-Girtu I, Helm MS, Badea RA, Rizzoli SO, Szedlacsek SE.
Sci Rep. 2020 May 20;10(1):8638. doi: 10.1038/s41598-020-65507-x.
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F.
Nanoscale. 2020 May 14;12(18):10226-10239. doi: 10.1039/d0nr00227e.
Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses.
Maus L, Lee C, Altas B, Sertel SM, Weyand K, Rizzoli SO, Rhee J, Brose N, Imig C, Cooper BH.
Cell Rep. 2020 Mar 17;30(11):3632-3643.e8. doi: 10.1016/j.celrep.2020.02.083.
The long noncoding RNA neuroLNC regulates presynaptic activity by interacting with the neurodegeneration-associated protein TDP-43.
Keihani S, Kluever V, Mandad S, Bansal V, Rahman R, Fritsch E, Gomes LC, Gärtner A, Kügler S, Urlaub H, Wren JD, Bonn S, Rizzoli SO, Fornasiero EF.
Sci Adv. 2019 Dec 18;5(12):eaay2670. doi: 10.1126/sciadv.aay2670. eCollection 2019 Dec.
Supersaturated proteins are enriched at synapses and underlie cell and tissue vulnerability in Alzheimer’s disease.
Freer R, Sormanni P, Ciryam P, Rammner B, Rizzoli SO, Dobson CM, Vendruscolo M.
Heliyon. 2019 Nov 19;5(11):e02589. doi: 10.1016/j.heliyon.2019.e02589. eCollection 2019 Nov.
A mass spectrometry workflow for measuring protein turnover rates in vivo.
Alevra M, Mandad S, Ischebeck T, Urlaub H, Rizzoli SO, Fornasiero EF.
Nat Protoc. 2019 Nov 4. doi: 10.1038/s41596-019-0222-y. [Epub ahead of print]
Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.
Reshetniak S, Rizzoli SO.
Front Synaptic Neurosci. 2019 Aug 23;11:23. doi: 10.3389/fnsyn.2019.00023. eCollection 2019. Review.
Pathological changes are associated with shifts in the employment of synonymous codons at the transcriptome level.
Fornasiero EF, Rizzoli SO.
BMC Genomics. 2019 Jul 9;20(1):566. doi: 10.1186/s12864-019-5921-9.
Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.
Richter KN, Patzelt C, Phan NTN, Rizzoli SO.
Sci Rep. 2019 Jun 25;9(1):9231. doi: 10.1038/s41598-019-45729-4.
GFP nanobodies reveal recently-exocytosed pHluorin molecules.
Seitz KJ, Rizzoli SO.
Sci Rep. 2019 May 23;9(1):7773. doi: 10.1038/s41598-019-44262-8.
A MICOS-TIM22 Association Promotes Carrier Import into Human Mitochondria.
Callegari S, Müller T, Schulz C, Lenz C, Jans DC, Wissel M, Opazo F, Rizzoli SO, Jakobs S, Urlaub H, Rehling P, Deckers M.
J Mol Biol. 2019 May 17. pii: S0022-2836(19)30284-0. doi: 10.1016/j.jmb.2019.05.015. [Epub ahead of print]
Fluorinated Nanobodies for Targeted Molecular Imaging of Biological Samples using Nanoscale Secondary Ion Mass Spectrometry.
Kabatas S, Agüi-Gonzalez P, Hinrichs R, Jähne S, Opazo F, Diederichsen U, Rizzoli SO, Phan NTN.
J. Anal. At. Spectrom., 2019 Apr 24; 34, 1083. DOI: 10.1039/c9ja00117d.
A practical guide to optimization in X10 expansion microscopy.
Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG.
Nat Protoc. 2019 Mar;14(3):832-863. doi: 10.1038/s41596-018-0117-3.
Boron-Containing Probes for Non-Optical High-Resolution Imaging of Biological Samples.
Kabatas S, Agüi-Gonzales P, Saal KA, Jähne S, Opazo F, Rizzoli SO, Phan TNN.
Angew Chem Int Ed Engl. 2019 Mar 11;58(11):3438-3443. doi: 10.1002/anie.201812032. Epub 2019 Feb 6.
Boron-Containing Probes for Non-Optical High-Resolution Imaging of Biological Samples.
Kabatas S, Agüi-Gonzales P, Saal KA, Jähne S, Opazo F, Rizzoli SO, Phan TNN.
Angew Chem 2019 Mar 11;131(11):3476-3481. doi:10.1002/anie.201812032. Epub 2019 Jan 7.
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F.
MAbs. 2019 Feb/Mar;11(2):305-321. doi: 10.1080/19420862.2018.1551675. Epub 2018 Dec 28.
Combined Use of Unnatural Amino Acids Enables Dual-Color Super-Resolution Imaging of Proteins via Click Chemistry.
Saal KA, Richter F, Rehling P, Rizzoli SO.
ACS Nano. 2018 Dec 26;12(12):12247-12254. doi: 10.1021/acsnano.8b06047. Epub 2018 Dec 13.
The codon sequences predict protein lifetimes and other parameters of the protein life cycle in the mouse brain.
Mandad S, Rahman RU, Centeno PT, Vidal RO, Wildhagen H, Rammner B, Keihani S, Opazo F, Urban I, Ischebeck T, Kirli K, Benito E, Fischer A, Yousefi RY, Dennerlein S, Rehling P, Feussner I, Urlaub H, Bonn S, Rizzoli SO, Fornasiero EF.
Sci Rep. 2018 Nov 15;8(1):16913. doi: 10.1038/s41598-018-35277-8.
Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions.
Fornasiero EF, Mandad S, Wildhagen H, Alevra M, Rammner B, Keihani S, Opazo F, Urban I, Ischebeck T, Sakib MS, Fard MK, Kirli K, Centeno TP, Vidal RO, Rahman RU, Benito E, Fischer A, Dennerlein S, Rehling P, Feussner I, Bonn S, Simons M, Urlaub H, Rizzoli SO.
Nat Commun. 2018 Oct 12;9(1):4230. doi: 10.1038/s41467-018-06519-0.
Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins.
Richter KN, Wildhagen H, Helm MS, Ußling JE, Schikorski T, Rizzoli SO.
Sci Rep. 2018 Oct 4;8(1):14838. doi: 10.1038/s41598-018-33130-6.
X10 expansion microscopy enables 25-nm resolution on conventional microscopes.
Truckenbrodt S, Maidorn M, Crzan D, Wildhagen H, Kabatas S, Rizzoli SO.
EMBO Rep. 2018 Sep;19(9). pii: e45836. doi: 10.15252/embr.201845836. Epub 2018 Jul 9.
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO.
EMBO J. 2018 Aug 1;37(15). pii: e98044. doi: 10.15252/embj.201798044. Epub 2018 Jun 27.
WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.
Mentel M, Ionescu AE, Puscalau-Girtu I, Helm MS, Badea RA, Rizzoli SO, Szedlacsek SE.
Sci Rep. 2018 Feb 13;8(1):2910. doi: 10.1038/s41598-018-21155-w.
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D’Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, Richter F, Coy-Vergara J, Coceano G, Boyden ES, Duncan RR, Hell SW, Lauterbach MA, Lehnart SE, Moser T, Outeiro T, Rehling P, Schwappach B, Testa I, Zapiec B, Rizzoli SO.
EMBO J. 2018 Jan 4;37(1):139-159. doi: 10.15252/embj.201695709. Epub 2017 Nov 16.
Hypoxia-stimulated membrane trafficking requires T-plastin.
Wottawa M, Naas S, Böttger J, van Belle GJ, Möbius W, Revelo NH, Heidenreich D, von Ahlen M, Zieseniss A, Kröhnert K, Lutz S, Lenz C, Urlaub H, Rizzoli SO, Katschinski DM.
Acta Physiol (Oxf). 2017 Sep;221(1):59-73. doi: 10.1111/apha.12859. Epub 2017 Mar 23.
Review of combined isotopic and optical nanoscopy.
Richter KN, Rizzoli SO, Jähne S, Vogts A, Lovric J.
Neurophotonics. 2017 Apr;4(2):020901. doi: 10.1117/1.NPh.4.2.020901. Epub 2017 Apr 22. Review.
ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.
Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N.
J Cell Biol. 2017 Apr 3;216(4):1143-1161. doi: 10.1083/jcb.201606086. Epub 2017 Mar 6.
Correction: ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.
Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N.
J Cell Biol. 2017 Apr 3;216(4):1205. doi: 10.1083/jcb.20160608603092017c. Epub 2017 Mar 13. No abstract available.
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO.
Curr Protoc Neurosci. 2016 Jan 4;74:2.25.1-2.25.21. doi: 10.1002/0471142301.ns0225s74.
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, Hagemeyer N, Dittrich M, Rossetti MF, Kröhnert K, Hannke K, Boretius S, Zeug A, Höschen C, Dandekar T, Dere E, Neher E, Rizzoli SO, Nave KA, Sirén AL, Ehrenreich H.
Mol Psychiatry. 2016 Jan 26. doi: 10.1038/mp.2015.212. [Epub ahead of print]
Editorial: Molecular Nanomachines of the Presynaptic Terminal.
Rizzoli SO, Tabares L.
Front Synaptic Neurosci. 2016 Aug 25;8:27. doi: 10.3389/fnsyn.2016.00027. eCollection 2016. No abstract available.PMID: 27610081
Tools and limitations to study the molecular composition of synapses by fluorescence microscopy.
Maidorn M, Rizzoli SO, Opazo F.
Biochem J. 2016 Oct 15;473(20):3385-3399. Review.
Optical dissection of experience-dependent pre- and postsynaptic plasticity in the Drosophila brain.
Pech U, Revelo NH, Seitz KJ, Rizzoli SO, Fiala A.
Cell Rep. 2015 Mar 31;10(12):2083-95. doi: 10.1016/j.celrep.2015.02.065. Epub 2015 Mar 26.[Epub ahead of print]
Super-Resolution Microscopy of Cerebrospinal Fluid Biomarkers as a Tool for Alzheimer’s Disease Diagnostics.
Zhang WI, Antonios G, Rabano A, Bayer TA, Schneider A, Rizzoli SO.
J Alzheimers Dis. 2015 Apr 16. [Epub ahead of print]
Secondary-ion mass spectrometry of genetically encoded targets.
Vreja IC, Kabatas S, Saka SK, Kröhnert K, Höschen C, Opazo F, Diederichsen U, Rizzoli SO.
Angew Chem Int Ed Engl. 2015 May 4;54(19):5784-8. doi: 10.1002/anie.201411692. Epub 2015 Mar 17.
Secondary-ion mass spectrometry of genetically encoded targets.
Vreja IC, Kabatas S, Saka SK, Kröhnert K, Höschen C, Opazo F, Diederichsen U, Rizzoli SO.
Angew Chem 2015 May 4;127(19):5876-5880. doi: 10.1002/anie.201411692. Epub 2015 Mar 17.
The structure and function of presynaptic endosomes.
Jähne S, Helm M, Rizzoli SO.
Exp Cell Res. 2015 Jul 15;335(2):172-9. doi: 10.1016/j.yexcr.2015.04.017. Epub 2015 May 1. Review.
A contamination-insensitive probe for imaging specific biomolecules by secondary ion mass spectrometry.
Kabatas S, Vreja IC, Saka SK, Höschen C, Kröhnert K, Opazo F, Rizzoli SO, Diederichsen U.
Chem Commun (Camb). 2015 Aug 6;51(67):13221-4. doi: 10.1039/c5cc03895b.
Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies.
Vreja IC, Nikić I, Göttfert F, Bates M, Kröhnert K, Outeiro TF, Hell SW, Lemke EA, Rizzoli SO.
ACS Nano. 2015 Oct 28. [Epub ahead of print].
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung S, Maritzen T, Wichmann C, Jing Z, Neef A, Revelo NH, Al-Moyed H, Meese S, Wojcik SM, Panou I, Bulut H, Schu P, Ficner R, Reisinger E, Rizzoli SO, Neef J, Strenzke N, Haucke V, Moser T.
EMBO J. 2015 Nov 3;34(21):2686-702. doi: 10.15252/embj.201591885. Epub 2015 Oct 7.
FM dye photo-oxidation as a tool for monitoring membrane recycling in inner hair cells.
Kamin D, Revelo NH, Rizzoli SO, Yan A, Levy M.
PLoS One. 2014 Feb 5;9(2):e88353. doi: 10.1371/journal.pone.0088353. eCollection 2014.
Synaptic vesicle recycling: steps and principles.
Rizzoli SO.
EMBO J. 2014 Apr 16;33(8):788-822. doi: 10.1002/embj.201386357. Epub 2014 Mar 3.
Correlated optical and isotopic nanoscopy.
Saka SK, Vogts A, Kröhnert K, Hillion F, Rizzoli SO, Wessels JT.
Nat Commun. 2014 Apr 10;5:3664. doi: 10.1038/ncomms4664.
Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy.
Zhang WI, Röhse H, Rizzoli SO, Opazo F.
Microsc Res Tech. 2014 Apr 10. doi: 10.1002/jemt.22367.
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO.
J Cell Biol. 2014 May 26;205(4):591-606. doi: 10.1083/jcb.201402066.
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, Rizzoli SO.
Science. 2014 May 30;344(6187):1023-8. doi: 10.1126/science.1252884.
Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.
Saka SK, Honigmann A, Eggeling C, Hell SW, Lang T, Rizzoli SO.
Nat Commun. 2014 Jul 25;5:4509. doi: 10.1038/ncomms5509.
Systematic comparison of the effects of alpha-synuclein mutations on its oligomerization and aggregation.
Lázaro DF, Rodrigues EF, Langohr R, Shahpasandzadeh H, Ribeiro T, Guerreiro P, Gerhardt E, Kröhnert K, Klucken J, Pereira MD, Popova B, Kruse N, Mollenhauer B, Rizzoli SO, Braus GH, Danzer KM, Outeiro TF.
PLoS Genet. 2014 Nov 13;10(11):e1004741. doi: 10.1371/journal.pgen.1004741. eCollection 2014 Nov.
Spontaneous vesicle recycling in the synaptic bouton.
Truckenbrodt S, Rizzoli SO.
Front Cell Neurosci. 2014 Dec 8;8:409. doi: 10.3389/fncel.2014.00409. eCollection 2014. Review.
FM dye photoconversion for visualizing synaptic vesicles by electron microscopy.
Hoopmann P, Rizzoli SO, Betz WJ.
Cold Spring Harb Protoc. 2012 Jan 1;2012(1):84-6. doi: 10.1101/pdb.prot067611.
Imaging synaptic vesicle recycling by staining and destaining vesicles with FM dyes.
Hoopmann P, Rizzoli SO, Betz WJ.
Cold Spring Harb Protoc. 2012 Jan 1;2012(1):77-83. doi: 10.1101/pdb.prot067603. Review.
An RNA alternative to human transferrin: a new tool for targeting human cells.
Wilner SE, Wengerter B, Maier K, de Lourdes Borba Magalhães M, Del Amo DS, Pai S, Opazo F, Rizzoli SO, Yan A, Levy M.
Mol Ther Nucleic Acids. 2012 May 15;1:e21. doi: 10.1038/mtna.2012.14.
Super-resolution imaging prompts re-thinking of cell biology mechanisms: selected cases using stimulated emission depletion microscopy.
Saka S, Rizzoli SO.
Bioessays. 2012 May;34(5):386-95. doi: 10.1002/bies.201100080. Epub 2012 Mar 13.
Use-dependent inhibition of synaptic transmission by the secretion of intravesicularly accumulated antipsychotic drugs.
Tischbirek CH, Wenzel EM, Zheng F, Huth T, Amato D, Trapp S, Denker A, Welzel O, Lueke K, Svetlitchny A, Rauh M, Deusser J, Schwab A, Rizzoli SO, Henkel AW, Müller CP, Alzheimer C, Kornhuber J, Groemer TW.
Neuron. 2012 Jun 7;74(5):830-44. doi: 10.1016/j.neuron.2012.04.019.
The Aspergillus nidlans MAPK module AnSte11-Ste50-Ste7-Fus3 controls development and secondary metabolism.
Bayram Ö, Bayram ÖS, Ahmed YL, Maruyama J, Valerius O, Rizzoli SO, Ficner R, Irniger S, Braus GH.
Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18944-9. doi: 10.1073/pnas.1203306109. Epub 2012 Oct 25.
Imaging synaptic vesicle recycling using FM dyes.
Hoopmann P, Rizzoli SO, Betz WJ.
Book chapter in: Imaging in Neuroscience: A Laboratory Manual. Editors: Fritjof Helmchen.
Systematic heterogeneity of fractional vesicle pool sizes and release rates of hippocampal synapses.
Welzel O, Henkel AW, Stroebel AM, Jung J, Tischbirek CH, Ebert K, Kornhuber J, Rizzoli SO, Groemer TW.
Biophys J. 2011 Feb 2;100(3):593-601. doi: 10.1016/j.bpj.2010.12.3706.
A small pool of vesicles maintains synaptic activity in vivo.
Denker A, Bethani I, Kröhnert K, Körber C, Horstmann H, Wilhelm BG, Barysch SV, Kuner T, Neher E, Rizzoli SO.
Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):17177-82. doi: 10.1073/pnas.1112688108. Epub 2011 Sep 8.
The reserve pool of synaptic vesicles acts as a buffer for proteins involved in synaptic vesicle recycling.
Denker A, Kröhnert K, Bückers J, Neher E, Rizzoli SO.
Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):17183-8. doi: 10.1073/pnas.1112690108. Epub 2011 Sep 8.
Studying synaptic vesicle pools using photoconversion of styryl dyes.
Opazo F, Rizzoli SO.
J Vis Exp. 2010 Feb 15;(36). doi:pii: 1790. 10.3791/1790.
Structure and dynamics of a two-helix SNARE complex in live cells.
Halemani ND, Bethani I, Rizzoli SO, Lang T.
Traffic. 2010 Mar;11(3):394-404. doi: 10.1111/j.1600-0854.2009.01020.x. Epub 2009 Nov 19.
Membrane protein clusters at nanoscale resolution: more than pretty pictures.
Lang T, Rizzoli SO.
Physiology (Bethesda). 2010 Apr;25(2):116-24. doi: 10.1152/physiol.00044.2009. Review.
t-SNARE protein conformations patterned by the lipid microenvironment.
Rickman C, Medine CN, Dun AR, Moulton DJ, Mandula O, Halemani ND, Rizzoli SO, Chamberlain LH, Duncan RR.
J Biol Chem. 2010 Apr 30;285(18):13535-41. doi: 10.1074/jbc.M109.091058. Epub 2010 Jan 21.
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann U, Schäfer C, Riedel D, Jahn R, Rizzoli SO.
Microsc Res Tech. 2010 Jun;73(6):606-17. doi: 10.1002/jemt.20800.
A fluorescence-based in vitro assay for investigating early endosome dynamics.
Barysch SV, Jahn R, Rizzoli SO.
Nat Protoc. 2010 Jun;5(6):1127-37. doi: 10.1038/nprot.2010.84. Epub 2010 May 27.
Limited intermixing of synaptic vesicle components upon vesicle recycling.
Opazo F, Punge A, Bückers J, Hoopmann P, Kastrup L, Hell SW, Rizzoli SO.
Traffic. 2010 Jun;11(6):800-12. doi: 10.1111/j.1600-0854.2010.01058.x. Epub 2010 Mar 9.
Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.
Lauterbach MA, Keller J, Schönle A, Kamin D, Westphal V, Rizzoli SO, Hell SW.
J Biophotonics. 2010 Jul;3(7):417-24. doi: 10.1002/jbio.201000038.
High- and low-mobility stages in the synaptic vesicle cycle.
Kamin D, Lauterbach MA, Westphal V, Keller J, Schönle A, Hell SW, Rizzoli SO.
Biophys J. 2010 Jul 21;99(2):675-84. doi: 10.1016/j.bpj.2010.04.054.
The fate of synaptic vesicle components upon fusion.
Opazo F, Rizzoli SO.
Commun Integr Biol. 2010 Sep;3(5):427-9. doi: 10.4161/cib.3.5.12132.
Synaptic vesicle pools: an update.
Denker A, Rizzoli SO.
Front Synaptic Neurosci. 2010 Oct 5;2:135. doi: 10.3389/fnsyn.2010.00135.
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R.
J Neurosci. 2010 Oct 6;30(40):13441-53. doi: 10.1523/JNEUROSCI.0907-10.2010.
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO.
Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19055-60. doi: 10.1073/pnas.1007037107. Epub 2010 Oct 18.
The same synaptic vesicles drive active and spontaneous release.
Wilhelm BG, Groemer TW, Rizzoli SO.
Nat Neurosci. 2010 Dec;13(12):1454-6. doi: 10.1038/nn.2690.
The mobility of synaptic vesicles.
Kamin D, Rizzoli SO.
Neuroforum 15:84-92.
Video-rate far-field optical nanoscopy dissects synaptic vesicle movement.
Westphal V*, Rizzoli SO*, Lauterbach MA, Kamin D, Jahn R, Hell SW.
*equal first authors
Science. 2008 Apr 11;320(5873):246-9. doi: 10.1126/science.1154228. Epub 2008 Feb 21.
Dual-color STED microscopy at 30-nm focal-plane resolution.
Meyer L, Wildanger D, Medda R, Punge A, Rizzoli SO, Donnert G, Hell SW.
Small. 2008 Aug;4(8):1095-100. doi: 10.1002/smll.200800055. No abstract available.
3D reconstruction of high-resolution STED microscope images.
Punge A, Rizzoli SO, Jahn R, Wildanger JD, Meyer L, Schönle A, Kastrup L, Hell SW.
Microsc Res Tech. 2008 Sep;71(9):644-50. doi: 10.1002/jemt.20602.
SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO.
Mol Biol Cell. 2008 Dec;19(12):5327-37. doi: 10.1091/mbc.E08-05-0457. Epub 2008 Oct 8.
Two-color far-field fluorescence nanoscopy.
Donnert G, Keller J, Wurm CA, Rizzoli SO, Westphal V, Schönle A, Jahn R, Jakobs S, Eggeling C, Hell SW.
Biophys J. 2007 Apr 15;92(8):L67-9. Epub 2007 Feb 16.
The specificity of SNARE pairing in biological membranes is mediated by both proof-reading and spatial segregation.
Bethani I, Lang T, Geumann U, Sieber JJ, Jahn R, Rizzoli SO.
EMBO J. 2007 Sep 5;26(17):3981-92. Epub 2007 Aug 23.
Endocytosis in neurons: editorial introduction and overview.
Jahn R, Rizzoli SO.
Traffic. 2007 Sep;8(9):1121-2. Epub 2007 Jul 20. No abstract available.
Kiss-and-run, collapse and ‚readily retrievable‘ vesicles.
Rizzoli SO, Jahn R.
Traffic. 2007 Sep;8(9):1137-44. Epub 2007 Jul 20. Review.
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
Brandhorst D*, Zwilling D*, Rizzoli SO*, Lippert U, Lang T, Jahn R.
*equal first authors
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2701-6. Epub 2006 Feb 9.
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig KI*, Rizzoli SO*, Westphal V, Jahn R, Hell SW.
*equal first authors
Nature. 2006 Apr 13;440(7086):935-9.
Macromolecular-scale resolution in biological fluorescence microscopy.
Donnert G, Keller J, Medda R, Andrei MA, Rizzoli SO, Lührmann R, Jahn R, Eggeling C, Hell SW.
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11440-5. Epub 2006 Jul 24.
Mobility of synaptic vesicles in different pools in resting and stimulated frog motor nerve terminals.
Gaffield MA, Rizzoli SO, Betz WJ.
Neuron. 2006 Aug 3;51(3):317-25.
Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.
Rizzoli SO, Bethani I, Zwilling D, Wenzel D, Siddiqui TJ, Brandhorst D, Jahn R.
Traffic. 2006 Sep;7(9):1163-76.
Synaptic vesicle pools.
Rizzoli SO, Betz WJ.
Nat Rev Neurosci. 2005 Jan;6(1):57-69. Review.
Modulation of nicotinic acetylcholine and N-methyl-d-aspartate receptors by some Hymenopteran venoms.
Zalat S, Elbana S, Rizzoli SO, Schmidt JO, Mellor IR.
Toxicon. 2005 Sep 1;46(3):282-90.
The structural organization of the readily releasable pool of synaptic vesicles.
Rizzoli SO, Betz WJ.
Science. 2004 Mar 26;303(5666):2037-9.
Effects of wortmannin and latrunculin A on slow endocytosis at the frog neuromuscular junction.
Richards DA, Rizzoli SO, Betz WJ.
J Physiol. 2004 May 15;557(Pt 1):77-91. Epub 2004 Mar 5.
Imaging synaptic vesicle dynamics with styryl dyes
Rizzoli SO, Becherer U, Angleson J, Betz WJ.
Book chapter in: Imaging In Neuroscience and Development. Editors: Rafael Yuste and Editors: Rafael Yuste and Athur Konnerth. Cold Spring Harbor Laboratory Press.
Neurobiology: All change at the synapse.
Rizzoli SO, Betz WJ.
Nature. 2003 Jun 5;423(6940):591-2. No abstract available.
Monitoring synaptic vesicle recycling in frog motor nerve terminals with FM dyes.
Rizzoli SO, Richards DA, Betz WJ.
J Neurocytol. 2003 Jun-Sep;32(5-8):539-49. Review.
Synaptic vesicle pools at the frog neuromuscular junction.
Richards DA, Guatimosim C, Rizzoli SO, Betz WJ.
Neuron. 2003 Jul 31;39(3):529-41.
Calcium rise in cultured neurons from medial septum elicits calcium waves in surrounding glial cells.
Rizzoli S, Sharma G, Vijayaraghavan S.
Brain Res. 2002 Dec 13;957(2):287-97.
Effects of 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one on synaptic vesicle cycling at the frog neuromuscular junction.
Rizzoli SO, Betz WJ.
J Neurosci. 2002 Dec 15;22(24):10680-9.
IgG isolated from LP-BM5 infected mouse brain activates ionotropic glutamate receptors.
Rizzoli S, Rogawski MA, Usherwood PN.
Neurobiol Dis. 2001 Dec;8(6):1069-81.
Solid phase synthesis and biological evaluation of enantiomerically pure wasp toxin analogues PhTX-343 and PhTX-12.
Strømgaard K, Bjørnsdottir I, Andersen K, Brierley MJ, Rizzoli S, Eldursi N, Mellor IR, Usherwood PN, Hansen SH, Krogsgaard-Larsen P, Jaroszewski JW.
Chirality. 2000 Feb;12(2):93-102.